Center for Bio-Image Informatics

Engineering, Biology and Computer Science, working together.

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Microscope usage and images uploaded, as stated in the logs until 2010-01-27

Name HG used # images uploaded # images in Bisque
Geoff Lewis 170.5 83 2076
Chris Banna 225.3 29 283 cmd:4256
Gabe Luna 174 50 2857
Gabe/Patrick 10.3 0 0
Patrick 1.7 0 0
Ethan 27.8 0 0
Ken Linberg 6.3 0 0
Michael Veeman/Bill Smith 244.6 0 3
Benoit Maury 13.8 0 0
Dmitry Fedorov 25.2 0 112
Boguslaw/Kippin 3.6 0 1
Brian Ruttenberg 6.3 0 0
Chespik 1.0 0 0
Bage 19.9 0 0
Ba 1.0 0 0
Lga 3.0 0 0

Start Up Instructions

Shut Down Instructions

 

Laser Scanning Microscope

The Olympus FV1000 confocal system is the confocal scanning system that will allow for complete automated control of the scanning during mosaic scanning. The FV1000 is confocal system capable of a 4096x4096 scan, a useful feature for our initial low magnification scans. The system also has several important features: constant laser power feedback, ensuring constant and even laser power; automated variable laser power and PMT sensitivity at different depths to adjust for light scatter in thicker samples; up to 16 virtual channel acquisition through automated sequential filter changes; integrated FRET, FRAP, and FLIP control and analysis, and 3D rendering capability.

Microscope documentation:

  • Start Up / Shut Down Instructions (PDF)