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The Olympus FV1000 confocal system is the confocal scanning system that will allow for complete automated control of the scanning during mosaic scanning. The FV1000 is confocal system capable of a 4096x4096 scan, a useful feature for our initial low magnification scans. The system also has several important features: constant laser power feedback, ensuring constant and even laser power; automated variable laser power and PMT sensitivity at different depths to adjust for light scatter in thicker samples; up to 16 virtual channel acquisition through automated sequential filter changes; integrated FRET, FRAP, and FLIP control and analysis, and 3D rendering capability.
Microscope documentation:
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