Center for Bio-Image Informatics

Engineering, Biology and Computer Science, working together.

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bioView3D is an open source and cross-platform application intended for biologists to visualize 3D stack (laser scanning confocal, etc.) imagery. It runs on Windows, MacOS X and Linux.


  • Cross-platform with binaries for Windows, Mac, Linux
  • Reads many bio image and video formats
  • Reads meta-data from BioRad PIC, TIFF, Metamorph STK (uncompressed and LZW compressed), Fluoview TIFF, Carl Zeiss LSM 5, PSIA TIFF, Nanoscope II/III
  • Has two modes of rendering: textures and voxels
  • On-the-fly 3D visualization (mapping/enhancement) of multi-channel data
  • Export of fly-over video to several popular formats: QuickTime, WMV, AVI, Flash, MPEG1/2/4, etc.
  • Visualization of graphical annotations: XML GObjects


Please, refer to our paper if you use this software.


Source code

Examples of video export