Understanding the mechanisms behind the loss and recovery of vision following retinal detachment and reattachment has been the focus of retinal biologists for many years. Analysis is usually done by comparing normal to detached retinas using sections and wholemounts, and considering the difference between two patterns.
Understanding the mechanisms behind the loss and recovery of vision following retinal detachment and reattachment has been the focus of retinal biologists for many years. Analysis is usually done by comparing normal to detached retinas using sections and wholemounts, and considering the difference between two patterns.
The most common method for determining cellular and subcellular patterns through fluorescence microscope images, which are generated by emitted fluorescence light (in essence "dark field"imaging) and collected by confocal imaging. An antibody is used to visualize a special cell shape and protein distributions in the retina.
Data can be collected from sections or from wholemounts
- A retinal section can be viewed from a vertical section of retina.
- A retinal wholemount can be viewed from either surface and then optically sectioned.
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